Method Development

Alongside using techniques already published, we also develop new methods to answer biologically-relevant questions. Some of the advances we have made include a new super-resolution technique to study proteins in live cells, a hyperspectral fluorescence lifetime microscope, and microfluidic technology for fluid handling. We've also worked with other researchers to test a series of new organic fluorophores for single-molecule imaging.

Team members

• Dylan George
• Beccy Saleeb
• Zuzanna Konieczna

Collaborators

• Gareth Williams
• Marc Vendrell
• Annalisa Pastore
• Gian Tartaglia

Selected Recent Publications

1. LIVE-PAINT allows super-resolution microscopy inside living cells using reversible peptide-protein interactions.

   Oi, C., Gidden, Z., Holyoake, L., Kantelberg, O., Mochrie, S., Horrocks, M.H.*, Regan, L.*

   Communications Biology, 3, 2020. http://dx.doi.org/10.1038/s42003-020-01188-6

2. ASYN-CONA, a novel bead-based assay for detecting early stage alpha-synuclein aggregation.

   Pérez-Pi, I., Evans, D.A., Horrocks, M.H., Pham, N.A., Dolt, K.S., Koszela, J., Kunath, T., Auer, M.

   Analytical Chemistry, 91, 9, 5582-5590, 2019. http://dx.doi.org/10.1021/acs.analchem.8b03842

3. SCOTfluors: Small, Conjugatable, Orthogonal and Tunable Fluorophores for in vivo Imaging of Cell Metabolism.

   Benson, S., Fernandez, A., Barth, N., de Moliner, F., Horrocks, M.H., Herrington, S., Abad, J.L., Delgado, A., Kelly, L., Chang, Z., Feng, Y., Nishiura, M., Hori, Y., Kikuchi, K., Vendrell, M.

   Angewandte Chemie, 58,6911–6915, 2019. http://dx.doi.org/10.1002/anie.201900465

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